'LazyData' is specified without a 'data' directory
Running examples in 'crispRdesignR-Ex.R' failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    x
 1. +-crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. | \-stringr::str_replace_all(revsetPAM, "N", ".")
 3. |   \-stringr:::check_lengths(string, pattern, replacement)
 4. |     \-vctrs::vec_size_common(...)
 5. \-vctrs:::stop_scalar_type(...)
 6.   \-vctrs:::stop_vctrs(...)
 7.     \-rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
'LazyData' is specified without a 'data' directory
Running examples in ‘crispRdesignR-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(...)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
Namespaces in Imports field not imported from:
  ‘DT’ ‘gbm’
  All declared Imports should be used.
'LazyData' is specified without a 'data' directory
Running examples in ‘crispRdesignR-Ex.R’ failed
The error most likely occurred in:

> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(...)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
'LazyData' is specified without a 'data' directory
Running examples in 'crispRdesignR-Ex.R' failed
The error most likely occurred in:

> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(...)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
'LazyData' is specified without a 'data' directory
Running examples in ‘crispRdesignR-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(...)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
'LazyData' is specified without a 'data' directory
Running examples in ‘crispRdesignR-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(`<fn>`(`<DNAStrng>`), "string", `<env>`)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
Package suggested but not available for checking: ‘BSgenome.Scerevisiae.UCSC.sacCer2’
Namespaces in Imports field not imported from:
  ‘DT’ ‘gbm’
  All declared Imports should be used.
'LazyData' is specified without a 'data' directory
Package suggested but not available for checking: ‘BSgenome.Scerevisiae.UCSC.sacCer2’
Namespaces in Imports field not imported from:
  ‘DT’ ‘gbm’
  All declared Imports should be used.
'LazyData' is specified without a 'data' directory
'LazyData' is specified without a 'data' directory
Running examples in 'crispRdesignR-Ex.R' failed
The error most likely occurred in:

> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    ▆
 1. ├─crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. │ └─stringr::str_replace_all(revsetPAM, "N", ".")
 3. │   └─stringr:::check_lengths(string, pattern, replacement)
 4. │     └─vctrs::vec_size_common(...)
 5. └─vctrs:::stop_scalar_type(`<fn>`(`<DNAStrng>`), "string", `<env>`)
 6.   └─vctrs:::stop_vctrs(...)
 7.     └─rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted
Package suggested but not available for checking: ‘BSgenome.Scerevisiae.UCSC.sacCer2’
Namespaces in Imports field not imported from:
  ‘DT’ ‘gbm’
  All declared Imports should be used.
'LazyData' is specified without a 'data' directory
Package suggested but not available for checking: ‘BSgenome.Scerevisiae.UCSC.sacCer2’
Namespaces in Imports field not imported from:
  ‘DT’ ‘gbm’
  All declared Imports should be used.
'LazyData' is specified without a 'data' directory
'LazyData' is specified without a 'data' directory
Running examples in 'crispRdesignR-Ex.R' failed
The error most likely occurred in:

> ### Name: getofftargetdata
> ### Title: Off Target Data Frame Creation
> ### Aliases: getofftargetdata
> 
> ### ** Examples
> 
> 
> ## Quick example without off-target searching or annotation
> ## First generate data with the sgRNA_Design Function
> testseq <- "GGCAGAGCTTCGTATGTCGGCGATTCATCTCAAGTAGAAGATCCTGGTGCAGTAGG"
> usergenome <- "placeholder"
> gtfname <- "placeholder"
> alldata <- sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
Searching sequence for possible target sites
Error in `stringr::str_replace_all()`:
! `string` must be a vector, not a <DNAString> object.
Backtrace:
    x
 1. +-crispRdesignR::sgRNA_design(testseq, usergenome, gtfname, calloffs = FALSE)
 2. | \-stringr::str_replace_all(revsetPAM, "N", ".")
 3. |   \-stringr:::check_lengths(string, pattern, replacement)
 4. |     \-vctrs::vec_size_common(...)
 5. \-vctrs:::stop_scalar_type(...)
 6.   \-vctrs:::stop_vctrs(...)
 7.     \-rlang::abort(message, class = c(class, "vctrs_error"), ..., call = vctrs_error_call(call))
Execution halted